EFFECT OF VOLUNTARY EXERCISE AND FOOD RESTRICTION IN RESPONSE TO LIPOPOLYSACCHARIDE IN HAMSTERS

We tested the hypothesis that voluntary running and moderate food rest riction alter the acute phase response (APR), one index of nonspecific immune function. Hamsters were kept sedentary or permitted to run and were fed ad libitum or had food restricted for 20 days and were then injected intraperitoneally with saline or lipopolysaccharide (LPS). Fe ver and circulating interleukin-6, serum amyloid A (SAA), serum iron, and cortisol were measured by biotelemetry, B-9 cell growth assay, ind irect enzyme-linked immunosorbent assay, colorimetric analysis, and ra dioimmunoassay, respectively. The febrile temperature; hypoferremia; a nd elevation of circulating interleukin-6, SAA, and cortisol after LPS injection were not altered by exercise. Because baseline temperatures were elevated in the exercised hamsters, the change in temperature in response to LPS was less than it was in the sedentary hamsters. Food restriction significantly decreased SAA and elevated cortisol after LP S injection and depressed the absolute temperature to which the core t emperature rose in response to LPS in one trial but not in another. Be cause food restriction depressed baseline temperatures, it also affect ed the change in temperature after LPS injection. The hypoferremic res ponse to LPS was inhibited in hamsters that were both food restricted and permitted to run. We conclude that exercise does not enhance the A PR to a low dose of LPS, whereas food restriction and the combination of exercise and food restriction depress some portions of the APR in h amsters.