TIME-DEPENDENT FLUORESCENCE DEPOLARIZATION ANALYSIS IN 3-DIMENSIONAL MICROSPECTROSCOPY

Authors
KOSHIOKA M SASAKI K MASUHARA H
Citation
M. Koshioka et al., TIME-DEPENDENT FLUORESCENCE DEPOLARIZATION ANALYSIS IN 3-DIMENSIONAL MICROSPECTROSCOPY, Applied spectroscopy, 49(2), 1995, pp. 224-228
Citations number
8
Categorie Soggetti
Instument & Instrumentation",Spectroscopy
Journal title
Applied spectroscopy → ACNP
ISSN journal
00037028
Volume
49
Issue
2
Year of publication
1995
Pages
224 - 228
Database
ISI
SICI code
0003-7028(1995)49:2<224:TFDAI3>2.0.ZU;2-C
Abstract
Three-dimensional space- and time-resolved fluorescence depolarization spectroscopy has been developed for elucidating picosecond rotational relaxation processes occurring in micrometer-sized volumes. Anisotrop y decays are obtained by a mw analytical method which is adapted to th e large-solid-angle characteristic of the microscope optics. System pa rameters introduced in this method were determined by measuring a refe rence sample. The fluorescence dynamics of p-terphenyl in a thin liqui d layer was analyzed as a demonstration experiment.