DETERMINATION OF PHENOL AND CHLORINATED PHENOLIC-COMPOUNDS BASED ON APPO-BIOELECTRODE AND ITS INHIBITION

A polyphenol oxidase (PPO) enzyme electrode constructed by the electro polymerization of a pyrrole amphiphilic monomer-PPO mixture, previousl y adsorbed on a glassy carbon electrode, is applied for the direct amp erometric response of phenol, 3-chlorophenol and 4-chlorophenol. Furth ermore, the detection of 2-chlorophenol, several polychlorophenols and pentachlorophenol is carried out by an inhibition process of the bioe lectrode functioning. The mechanism of the bioelectrode inhibition is investigated in terms of enzymatic inhibition and polymer fouling. The capacities of this system for the resolution of mixtures of phenolic pollutants are also explored on the basis of the kinetic behavior of t he bioelectrode response.