DETERMINATION OF URINARY OXALATE USING BANANA OXALATE OXIDASE - COMPARISON WITH IMMOBILIZED ENZYME

Oxalate oxidase, isolated from banana fruit peel, is immobilized on va rious supports and the efficacies of these derivatives in determining urinary oxalate are compared. Banana oxalate oxidase, when complexed w ith its antiserum, is found to have higher specific activity, greater stability, broader range of pH profile and higher resistance to therma l inactivation. Oxalate oxidase, when immobilized in acrylamide membra ne strip, is found to be stable and easy to use in the determination o f urinary oxalate. These two preparations compare well with that of so luble enzyme in sensitivity, accuracy and reliability. However, both h ydrazide bead-linked oxalate oxidase and ethylene maleic anhydride der ivative of oxalate oxidase are found to be unsuitable for the determin ation of urinary oxalate.