From whole plants of Salpichroa origanifolia, collected before the flo
wering stage, three new withanolides were isolated and their structure
s established by spectroscopic and chemical methods. Two of them, salp
ichrolides B [2] and C [3], contain a six-membered aromatic D ring, wh
ile the third one, salpichrolide D [4], has a five-membered D ring wit
h a Delta(16)-15 ketone system.