P. Mahannop et al., IMMUNODIAGNOSIS OF HUMAN TRICHINOSIS AND IDENTIFICATION OF SPECIFIC ANTIGEN FOR TRICHINELLA-SPIRALIS, International journal for parasitology, 25(1), 1995, pp. 87-94
Crude antigens obtained from the infective stage larvae of Trichinella
spiralis were used in an ELISA for detecting IgG antibodies to T. spi
ralis in serum samples collected from three groups of individuals. The
individuals of the first group were parasitologically confirmed trich
inellosis patients, while those of group 2 were patients with other he
lminthiasis and group 3 were healthy, parasite-free individuals. The s
pecificity of the assay was 96.8% when performed on sera of groups 2 a
nd 3. Cross-reaction was observed with the sera of patients with capil
lariasis, gnathostomiasis, opisthorchiasis, and strongyloidiasis and o
pisthorchiasis with hookworm infection, The sensitivity of the test wa
s 100% when performed on sera of group 1, which were collected 57 days
after infection, Western blot analysis revealed that a specific antig
en for T. spiralis was a component of M(r) 109.