IDENTIFICATION AND CHARACTERIZATION OF SCHISTOSOMA-MANSONI ANTIGENS RECOGNIZED BY T-LYMPHOCYTES AND B-LYMPHOCYTES OF HUMANS WITH EARLY ACTIVE INTESTINAL AND OR URINARY SCHISTOSOMIASIS/
M. Alsherbiny et al., IDENTIFICATION AND CHARACTERIZATION OF SCHISTOSOMA-MANSONI ANTIGENS RECOGNIZED BY T-LYMPHOCYTES AND B-LYMPHOCYTES OF HUMANS WITH EARLY ACTIVE INTESTINAL AND OR URINARY SCHISTOSOMIASIS/, International journal for parasitology, 25(1), 1995, pp. 113-121
Schistosome antigens selected as vaccine candidates should induce in t
he majority of humans T and B cell-mediated immunity that results in p
rotection against infection. As a first step towards the identificatio
n of such antigens, we attempted to define and characterize the solubl
e adult Schistosoma mansoni worm antigen (SAWA) bands that are recogni
zed by serum antibodies and/or peripheral blood mononuclear cells of E
gyptian children with early active S. mansoni and/or S. haematobium in
fection, Considerable inter-subject variation was observed in the SAWA
bands recognized by antibodies and T lymphocytes, as demonstrated by
Western blotting and T tell Western assays, respectively. The humoral
response rate for the separated SAWA bands varied between 0% and 88% o
f infected subjects. The bands of 153, 144, 38 and 32 kDa reacted with
the sera of 60 to 88% of infected subjects but not with the sera of u
ninfected controls. The bands of 144, 38, 32 and 18 kDa elicited proli
ferative responses in the lymphocytes of 42-63% of infected subjects,
It was thus concluded that the SAWA bands of 144, 38 and 32 kDa are li
kely to carry T and B cell epitopes that could stimulate immune respon
ses in a majority of individuals. The selected bands (144, 38 and 32 k
Da) were found to include glycoproteins containing D-mannopyranosyl or
glycosyl residues, and respectively 62.5, 46 and 55% amino acids by w
eight, The amino acid molar ratios of these bands were completely diff
erent,