A 1.8-MB YAC CONTIG SPANNING 3 MEMBERS OF THE RECEPTOR TYROSINE KINASE GENE FAMILY (PDGFRA, KIT, AND FLK1) ON MOUSE CHROMOSOME-5

We constructed a yeast artificial chromosome (YAC) contig spanning the genes encoding Kit (Kit), the platelet-derived growth factor cu recep tor (Pdgfra), and fetal liver kinase 1 (Flk1), three members of a rece ptor tyrosine kinase gene family located in the central portion of mou se chromosome 5. The orientation of YAC clones and the extent of their overlap was determined by ''probe content mapping,'' that is, hybridi zation analysis of YAC clones using the available gene probes and YAC end sequences. For four YAC clones, which constitute a minimal set spa nning 1.8 Mb, a detailed restriction map was constructed. This map, in conjunction with the previously published long-range restriction map, indicates the order, the physical distances, and the relative transcr iptional orientations of the Pdgfra, Kit, and Flk1 genes. The YAC clon es and corresponding YAC end probes presented here provide an importan t resource for the molecular analysis of a cluster of developmental mu tations, namely dominant white spotting (W), patch (Ph), recessive spo tting (rs), and rump-white (Rw), associated with this chromosomal regi on. (C) 1995 Academic Press, Inc.