A HIGH-RESOLUTION INTEGRATED PHYSICAL, CYTOGENETIC, AND GENETIC-MAP OF HUMAN-CHROMOSOME-11 - DISTAL P13 TO PROXIMAL P15.1

We describe a detailed physical map of human chromosome 11, extending from the distal part of p13 through the entirety of p14 to proximal p1 5.1. The primary level of mapping is based on chromosome breakpoints t hat divide the region into 20 intervals. At higher resolution YACs cov er approximately 12 Mb of the region, and in many places overlapping c osmids are ordered in contiguous arrays. The map incorporates 18 known genes, including precise localization of the GTF2H1 gene encoding the 62-kDa subunit of TFIIH. We have also localized four expressed sequen ces of unknown function. The physical map incorporates genetic markers that allow relationships between physical and genetic distance to be examined, and similarly includes markers from a radiation hybrid map o f 11. The cytogenetic location of cosmids has been examined on high-re solution banded chromosomes by fluorescence in situ hybridization, and FLpter values have been determined. The map therefore fully integrate s physical, genic, genetic, and cytogenetic information and should pro vide a robust framework for the rapid and accurate assignment of new m arkers at a high level of resolution in this region of 11p. (C) 1995 A cademic Press, Inc.