LOCALIZATION OF AN EPITHELIAL-SPECIFIC RECEPTOR KINASE (EDDR1) TO CHROMOSOME 6Q16

A protein receptor tyrosine kinase (EDDR1) has been isolated from a co mplementary DNA library of SKOV-3, an epithelial ovarian cancer cell l ine. The primary structure of the predicted amino acid sequence of the protein shows a novel N-terminal region that has homology to a factor VIII-like domain. The C-terminal catalytic domain has all of the cano nical sequence motifs of a receptor tyrosine kinase with homology to t he TRK-2H protein (49%), which suggests that it is a type II receptor. It is expressed in epithelial cells of several tissues. To determine the chromosomal localization of the gene, somatic cell hybrids were an alyzed by PCR amplification using oligonucleotide primers specific for EDDR1. Segregation was observed to a hybrid containing human chromoso me 6. Cosmids for EDDR1 were isolated from a human chromosome 6 cosmid library and were shown by fluorescence in situ hybridization to map t o 6q16. (C) 1995 Academic Press, Inc.