DETERMINATION OF GLUTAMINE IN MAMMALIAN-CELL CULTURES WITH A FLOW-INJECTION ANALYSIS WALL-JET ELECTRODE SYSTEM

A flow injection analysis/wall-jet electrode system coupled to a react or packed with controlled pore glass beads containing coimmobilized gl utaminase/glutamate oxidase was investigated for glutamine determinati on in mammalian cell cultures. In the reactor, glutamine was converted to hydrogen peroxide which was detected amperometrically at the wall- jet electrode controlled at +0.65 V (vs Ag/AgCl, saturated KCl). The s ystem was tested for its stability, linearity and precision of respons e: to glutamine. Finally, it was applied to determination of glutamine in various mammalian cell cultures.