STRESS-INDUCED SECRETION OF PRO-OPIOMELANOCORTIN-DERIVED PEPTIDES IN RATS - RELATIVE IMPORTANCE OF THE ANTERIOR AND INTERMEDIATE PITUITARY LOBES

Stress stimulates secretion of the pro-opiomelanocortin (POMC)-derived peptides adrenocorticotropic hormone (ACTH), beta-endorphin (beta-END ) and alpha-melanocyte-stimulating hormone (alpha-MSH) from the anteri or lobe (AL) and intermediate lobe (IL) of the pituitary gland. The se cretion of POMC-derived peptides from the AL and IL is differentially regulated and the relative contribution of the lobes may vary with the stimulus. We investigated (1) the relative importance of the AL and I L as source of POMC-derived peptides released in response to restraint and ether stress by selectively inhibiting the corticotropes of the A L by dexamethasone (DEX) or selectively inhibiting the melanotropes of the IL by bromocriptine (BR), and (2) whether beta-adrenergic blockad e by propranolol could be used to discriminate between the stress-indu ced effect on POMC secretion from the AL and IL as has previously been suggested. Selective inhibition of AL secretion by DEX totally blocke d the ACTH response to restraint and ether stress, but only partially inhibited the beta-END response. The alpha-MSH response to both stress ors was not affected by DEX. Conversely, selective inhibition of IL se cretion by BR totally blocked the alpha-MSH response to both stressors , partially inhibited the beta-END response but did not influence the ACTH response. In response to restraint stress, beta-END was secreted equally from the AL and IL, whereas the IL was the mast important sour ce of beta-END in response to ether stress. Blockade of beta-adrenergi c receptors with propranolol inhibited the beta-END- and alpha-MSH res ponses to restraint stress whereas the ACTH response was unaffected. T he secretary response of both ACTH, beta-END and alpha-MSH to ether st ress was inhibited by propranolol. We conclude, that (1) restraint- an d ether-stress-induced secretion of ACTH and alpha-MSH are of AL and I L origin, respectively, (2) beta-END secreted in response to restraint stress originates almost equally from the AL and IL, whereas the sour ce of beta-END in response to ether stress is mainly the IL, and (3) b eta-adrenergic blockade by propranolol cannot be used to discriminate between the AL and IL as the source of POMC peptides secreted in respo nse to stress.