INACTIVATION OF POLYMERASE INHIBITORS FOR MYCOBACTERIUM-TUBERCULOSIS DNA AMPLIFICATION IN SPUTUM BY USING CAPTURE RESIN

Authors
AMICOSANTE M RICHELDI L TRENTI G PAONE G CAMPA M BISETTI A SALTINI C
Citation
M. Amicosante et al., INACTIVATION OF POLYMERASE INHIBITORS FOR MYCOBACTERIUM-TUBERCULOSIS DNA AMPLIFICATION IN SPUTUM BY USING CAPTURE RESIN, Journal of clinical microbiology, 33(3), 1995, pp. 629-630
Citations number
10
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
3
Year of publication
1995
Pages
629 - 630
Database
ISI
SICI code
0095-1137(1995)33:3<629:IOPIFM>2.0.ZU;2-G
Abstract
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tu berculosis DNA in sputum samples. Use of an anion binding resin (GeneR eleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-posi tive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).