COLORIMETRIC MICROWELL PLATE HYBRIDIZATION ASSAY FOR DETECTION OF AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DNA FROM SPUTUM SAMPLES

Authors
CHO SN VANDERVLIET GME PARK S BAIK SH KIM SK CHONG Y KOLK AHJ KLATSER PR KIM JD
Citation
Sn. Cho et al., COLORIMETRIC MICROWELL PLATE HYBRIDIZATION ASSAY FOR DETECTION OF AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DNA FROM SPUTUM SAMPLES, Journal of clinical microbiology, 33(3), 1995, pp. 752-754
Citations number
16
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
3
Year of publication
1995
Pages
752 - 754
Database
ISI
SICI code
0095-1137(1995)33:3<752:CMPHAF>2.0.ZU;2-H
Abstract
We developed a colorimetric microwell plate hybridization assay (CoMPH A) for the specific detection of 5'-biotinylated amplified Mycobacteri um tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that th e CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.