THE RAT-LIVER ECTO-ATPASE C-CAM CDNA DETECTS INDUCTION OF CARCINOEMBRYONIC ANTIGEN BUT NOT THE MERCURIAL-INSENSITIVE ECTO-ATPASE IN HUMAN HEPATOMA LI-7A CELLS TREATED BY EPIDERMAL GROWTH-FACTOR AND CHOLERA-TOXIN
Af. Knowles, THE RAT-LIVER ECTO-ATPASE C-CAM CDNA DETECTS INDUCTION OF CARCINOEMBRYONIC ANTIGEN BUT NOT THE MERCURIAL-INSENSITIVE ECTO-ATPASE IN HUMAN HEPATOMA LI-7A CELLS TREATED BY EPIDERMAL GROWTH-FACTOR AND CHOLERA-TOXIN, Biochemical and biophysical research communications, 207(2), 1995, pp. 529-535
The rat liver ectoATPase has reportedly been cloned. The cDNA, a membe
r of the carcinomebryonic antigen (CEA) gene family, was shown to incr
ease aggregation of transfected cells, but ATPase activity was not eva
luated. Using this cDNA as a probe to clone the mercurial-insensitive
ectoATPase (MI-ectoATPase) of human hepatoma Li-7A cells, the cDNA obt
ained was that of CEA which has no ATPase activity. The probe also did
not detect increased transcription when MI-ectoATPase activity was in
duced in Li-7A cells. It is concluded that the ''rat liver ectoATPase
cDNA'' codes for a cell adhesion molecule but does not code for an ect
oATPase. It was also discovered that expression of four CEA transcript
s in Li-7A cells was markedly stimulated by a single growth modulator,
EGF, and was further stimulated by acAMP elevating agent, cholera tox
in. (C) 1995 Academic Press, Inc.