CLONING OF A CDNA CODING FOR PORCINE ZONA-PELLUCIDA GLYCOPROTEIN ZP1 AND ITS GENOMIC ORGANIZATION

The zona pellucida composed of three or four glycoproteins plays impor tant roles in fertilization. Our previous study showed that porcine ZP 1, one of the major glycoproteins of porcine zona pellucida, was divid ed into two components (porcine ZP4 and ZP2), and suggested it was a h omologue of mouse ZP2. In this paper we report the cloning of a cDNA f or porcine ZP1 and its genomic organization. The deduced amino acid se quence of porcine ZP1 shared a 54% and 63% identity with those of mous e and human ZP2, respectively. Genomic organization of porcine ZP1 was also similar to that of mouse ZP2. The transcript of porcine ZP1 gene was detected only in growing oocytes. (C) 1995 Academic Press, Inc.