TUMOR-NECROSIS-FACTOR-ALPHA AND INTERLEUKIN-6 SELECTIVELY REGULATE NEUTROPHIL FUNCTION IN-VITRO

The neutrophil is an important effector cell of the host response to s epsis. Tumor necrosis factor-alpha) (TNF-alpha), a cytokine mediator o f the septic response, is rapidly released following endotoxemia or gr am-negative bacteremia. Interleukin-g (IL-6) is another cytokine media tor of the host response to sepsis whose role is less well understood than that of TNF-alpha. It is known to be elevated in gram-negative se psis, where peak levels have been correlated with mortality. This stud y examined the effect of IL-6 alone and in combination with TNF-alpha on three neutrophil functions-CD18 adhesion receptor expression, phago cytosis, and superoxide anion generation. Neutrophils from human volun teers were incubated with amounts of IL-6 ranging from 10 to 1000 ng/m l. At a concentration of 1000 ng/ml, IL-6 increased neutrophil phagocy tosis of opsonized bacteria (826 +/- 255 x 10(3) MESF vs 552 +/- 103 M ESF, P < 0.05) and also increased neutrophil superoxide anion generati on (18.41 +/- 1.86 vs 12.6 nmol O-2(-)/10(8) PMN/10 min, P < 0.05). Le sser amounts of IL-6 had no effect on phagocytosis or superoxide gener ation. IL-6 did not increase neutrophil CD18 adhesion receptor express ion. Combining IL-6 with TNF-alpha at doses of 100 ng/ml and 100 U/ml, respectively, neutrophil phagocytosis (221 +/- 455 MESF vs 552 +/- 10 3 MESF) and superoxide generation (23.18 +/- 1.86 vs 12.6 nmol O-2(-)/ 10(6) PMN/10 min) were significantly (P < 0.05) increased above contro l by an amount similar to that seen with 1000 U/ml TNF-alpha alone. IL -6 and TNF-alpha combined did not further increase CD18 expression ove r that produced by 100 U/ml TNF-alpha alone. We conclude that IL-6 sel ectively influences neutrophil function so that, with regard to phagoc ytosis and superoxide generation, the response of neutrophils to a com bination of 100 ng/ml IL-6 and 100 U/ml TNF-alpha equals that produced by 1000 U/ml of TNF-alpha alone. This synergy between IL-6 and TNF-al pha may represent an important mechanism by which IL-6 potentiates the effect of TNF-alpha in vivo. (C) 1995 Academic Press, Inc.