SMALL-BOWEL TISSUE HIGH-ENERGY PHOSPHATE REGENERATION AFTER 7 HR OF COLD ISCHEMIC STORAGE - COMPARISON OF UNIVERSITY-OF-WISCONSIN AND EUROCOLLINS SOLUTIONS

As adenine nucleotide content has been shown to correlate with post-tr ansplant function of livers and hearts, it was the aim of our study to investigate the regeneration of rat small bowel tissue high-energy ph osphates after 7 hr of cold storage followed by incubation of everted small bowel sacs in normothermic oxygenated KHB for 1 hr. We compared the University of Wisconsin (UW) and the Eurocollins (EC) solutions. K rebs-Henseleit-bicarbonate buffer (KHB) was used to point out the effe ct of simple cold ischemic storage. After 7 hr of cold storage only sm all bowel stored in UW and EC solutions retained the capacity for almo st total regeneration of ATP necessary for optimal posttransplant func tion, whereas in the KHB group we found only minimal regeneration. A s imilar pattern was found for the energy charge. These data support the superiority of UW and EC solutions over simple cold storage in KHB fo r preservation of small bowel. (C) 1995 Academic Press,Inc.