USE OF LACTATE-DEHYDROGENASE TO EVALUATE THE ANTIVIRAL ACTIVITY AGAINST INFLUENZA-A VIRUS

Authors
WATANABE W SUDO K ASAWA S KONNO K YOKOTA T SHIGETA S
Citation
W. Watanabe et al., USE OF LACTATE-DEHYDROGENASE TO EVALUATE THE ANTIVIRAL ACTIVITY AGAINST INFLUENZA-A VIRUS, Journal of virological methods, 51(2-3), 1995, pp. 185-191
Citations number
12
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
51
Issue
2-3
Year of publication
1995
Pages
185 - 191
Database
ISI
SICI code
0166-0934(1995)51:2-3<185:UOLTET>2.0.ZU;2-C
Abstract
The detection of lactate dehydrogenase (LDH) can be used to evaluate e fficiently anti-influenza A virus agents. LDH levels in the virus-infe cted Madin-Darby canine kidney cell cultures were significantly higher than in controls, were in proportion to the degree of virus infection , and corresponded to a decrease in mitochondrial dehydrogenase activi ty as assayed using a tetrazolium colorimetric assay (MTT method). The EC(50) value and cytotoxicity of ribavirin, 3-deazaguanine, pyrazofur in, and carbodine against influenza A virus as measured by the LDH det ection method was equivalent to that derived by the MTT method.