EFFECT OF YOLK AND SUGAR LEVELS IN DILUTORS ON MOTILITY OF BUFFALO SPERMATOZOA DURING REFRIGERATION PRESERVATION

Murrah buffalo semen (initial motility 70% and above) was preserved at 5 degrees C up to 72 hr in milk, tris and sodium citrate dilutors wit h different levels of yolk and different concentrations of various sug ars and no glycerol. Motility in all the buffers varied in the presenc e of different yolk levels. Highest motility with 5% yolk was observed and it decreased as yolk concentration increased. During the first 24 hr of preservation tris was better over milk and citrate, but on subs equent hours of preservation milk was better and citrate was poorest a mong all the 3 buffers. Addition of sugars increased the motility in m ilk and tris but nor in citrate. Different sugars behaved differently in different buffers and lower level of sugars were slightly beneficia l over its higher level. During refrigeration storage, concentration o f yolk and type and levels of sugars are important factors in determin ing the preservation protection.