DISRUPTION OF THE GENE ENCODING THE 78-KILODALTON SUBUNIT OF THE PERIPHERAL ARM OF COMPLEX-I IN NEUROSPORA-CRASSA BY REPEAT INDUCED POINT MUTATION (RIP)
Taa. Harkness et al., DISRUPTION OF THE GENE ENCODING THE 78-KILODALTON SUBUNIT OF THE PERIPHERAL ARM OF COMPLEX-I IN NEUROSPORA-CRASSA BY REPEAT INDUCED POINT MUTATION (RIP), Current genetics, 27(4), 1995, pp. 339-350
We have used the procedure of sheltered RIP to generate mutants of the
78-kDa protein of the peripheral arm of Neurospora crassa complex I.
The nuclei containing the mutations were initially isolated as one com
ponent of a heterokaryon but subsequent analysis showed that nuclei co
ntaining null alleles of the gene could be propagated as homokaryons.
This demonstrates that the gene does not serve an essential function.
Sequence analysis of one allele shows that 61 transition mutations wer
e created resulting in 39 amino-acid changes including the introductio
n of four stop codons. Mutant strains grow at a slower rate than wild-
type and exhibit a decrease in the production of conidia. Electron par
amagnetic spectroscopy of mutant mitochondria suggest that they are de
ficient in Fe-S clusters N-1, N-3, and N-4.