EXPRESSION OF A YEAST GENE CAN BE BLOCKED BY INSERTION OF SHORT YEASTDNA FRAGMENTS BETWEEN A UAS AND THE TATA BOX

Authors
VINCENT O GANCEDO JM
Citation
O. Vincent et Jm. Gancedo, EXPRESSION OF A YEAST GENE CAN BE BLOCKED BY INSERTION OF SHORT YEASTDNA FRAGMENTS BETWEEN A UAS AND THE TATA BOX, Current genetics, 27(4), 1995, pp. 387-389
Citations number
15
Categorie Soggetti
Genetics & Heredity
Journal title
Current genetics → ACNP
ISSN journal
01728083
Volume
27
Issue
4
Year of publication
1995
Pages
387 - 389
Database
ISI
SICI code
0172-8083(1995)27:4<387:EOAYGC>2.0.ZU;2-F
Abstract
We have constructed a plasmid, pOV10, which facilitates the introducti on of putative upstream activating sequences (UAS) or upstream repress ing sequences (URS) from yeast genes into plasmids containing CYC1-lac Z fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block beta-galactosidase expression. It is suggested that thi s block is related to the formation of nucleosomes on the DNA.