INDUCTION OF APOPTOSIS BY ENEDIYNE ANTITUMOR ANTIBIOTIC C1027 IN HL-60 HUMAN PROMYELOCYTIC LEUKEMIA-CELLS

C1027, a new macromolecular antitumor antibiotic with an enediyne chro mophore, displays extremely potent cytotoxicity against cancer cells. HL-60 human promyelocytic leukemia cells treated with C1027 (0.1-10 nM ) for 2 hours resulted in morphological changes, including Hoechst 333 42-stained condensed nuclei, condensation of nuclear chromatin, and nu clear fragmentation. Agarose gel electrophoresis of C1027-treated HL-6 0 cells showed a typical ladder-like pattern of DNA fragments. In addi tion, the apoptotic DNA peak of propidium iodide-stained nuclei was re vealed by flow cytometry. Treatment of HL-60 cells with C1027 (5 nM) i nduced apoptosis in up to 79% of the cells. The results suggest that C 1027 may exert antitumor activity by triggering apoptosis. (C) 1995 Ac ademic Press, Inc.