IN-VITRO PLANT-REGENERATION FROM LEAF BASE AND MESOCOTYL CULTURES OF ECHINOCHLOA-COLONA

Leaf base and mesocotyl explants derived from in vitro-grown seedlings of Echinochloa colona were cultured on Murashige and Skoog's (MS) med ium containing various concentrations of benzyladenine (BA), alpha-nap hthaleneacetic acid (NAA) and kinetin. Leaf base and mesocotyl segment s exhibited optimal morphogenetic response by using 6.66 mu M BA with 2.68 mu M NAA. Induction of rooting from regenerated shoots was readil y achieved in half strength MS medium without organics and growth regu lators. Histological studies revealed the sequence of shoot bud regene ration in the monocot system. The in vitro-raised plants were establis hed in chromite minewaste.