ASSESSMENT OF MICROINJECTION FOR INTRODUCING DNA INTO UNINUCLEAR MICROSPORES OF RAPESEED

Authors
JONESVILLENEUVE E HUANG B PRUDHOMME I BIRD S KEMBLE R HATTORI J MIKI B
Citation
E. Jonesvilleneuve et al., ASSESSMENT OF MICROINJECTION FOR INTRODUCING DNA INTO UNINUCLEAR MICROSPORES OF RAPESEED, Plant cell, tissue and organ culture, 40(1), 1995, pp. 97-100
Citations number
11
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
40
Issue
1
Year of publication
1995
Pages
97 - 100
Database
ISI
SICI code
0167-6857(1995)40:1<97:AOMFID>2.0.ZU;2-N
Abstract
Approximately 2,000 embryogenic uninuclear microspores of rapeseed (Br assica napus) cv. Topas were intranuclearly injected with a chimaeric beta-glucuronidase (Escherichia coli Uid A) gene. Stable integration h ad not occurred among 55 plants that were regenerated. Coinjection of the dye Lucifer Yellow and detection of injected DNA by the polymerase chain reaction revealed high frequencies of transfer. However, the am ount of DNA injected was less than 20 copies, which may have been insu fficient for stable transformation of microspores.