ISCHEMIA-INDUCED CHANGE IN CLATHRIN PRECEDING DELAYED NEURONAL DEATH

THE mechanism underlying the change in clathrin immunohistochemistry p receding delayed neuronal death (DND) was studied in gerbils. The isch aemic change observed with chc5.9 anti-clathrin antibody in hippocampa l CA1 was initially ameliorated by pentobarbital, which blocks DND, bu t 1 day after ischaemia, no change in the immunoreactivity of the SDS- denatured clathrin molecule was detected by Western blotting and no ch ange in the clathrin content in CA1 was detected by SDS-PAGE. No ischa emia-induced change in immunohistochemistry was observed with another monoclonal anti-clathrin antibody, X-22. The above results imply that some modifications that affect the structure of clathrin molecules aro und the chc5.9 specific epitope may be a crucial step in the course of DND.