Jj. Gray, AVIDITY OF EBV VCA-SPECIFIC IGG ANTIBODIES - DISTINCTION BETWEEN RECENT PRIMARY INFECTION, PAST INFECTION AND REACTIVATION, Journal of virological methods, 52(1-2), 1995, pp. 95-104
Citations number
28
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A commercial Epstein-Barr virus (EBV) antivirus capsid antigen (VCA) I
gG antibody ELISA and an 'in-house' EBV VCA IgG immunofluorescence ant
ibody assay (IFA) were used to detect EBV VCA IgG antibodies in 100 se
rum samples collected from organ transplant recipients and immunocompe
tent individuals. The avidity of EBV VCA IgG antibodies was determined
in the IFA and ELISA using the mild reducing agent 8 M urea to remove
low avidity antibodies. The samples were collected from patients who
had previously been identified with a primary EBV infection, a reactiv
ation of latent infection or evidence of a past EBV infection by means
of EBV-specific serology. Using the ELISA, the antibody avidity was l
ow in samples collected from patients with recent EBV infection and hi
gh in samples collected from patients with a past infection or a react
ivation. There was a statistically significant difference of means (P
< 0.001) of percentage reduction in optical density values, measured i
n the presence of 8 M urea, obtained with samples collected from patie
nts with recent infection compared with samples from patients with a p
ast infection or a reactivation of latent infection.