DETECTION OF EPSTEIN-BARR VIRUS-SPECIFIC ANTIBODIES BY MEANS OF BACULOVIRUS-EXPRESSED EBV GP125

Authors
SANCHEZMARTINEZ D PATTON JL STEWART JA PELLETT PE
Citation
D. Sanchezmartinez et al., DETECTION OF EPSTEIN-BARR VIRUS-SPECIFIC ANTIBODIES BY MEANS OF BACULOVIRUS-EXPRESSED EBV GP125, Journal of virological methods, 52(1-2), 1995, pp. 145-153
Citations number
28
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
52
Issue
1-2
Year of publication
1995
Pages
145 - 153
Database
ISI
SICI code
0166-0934(1995)52:1-2<145:DOEVAB>2.0.ZU;2-R
Abstract
A major antigenic component of the Epstein-Barr virus viral capsid ant igen (VCA) complex is the glycoprotein, gp125. Baculovirus-expressed g p125 reacted with Epstein-Barr virus IgG antibodies in a panel of 44 s erum specimens using an immunoblot assay with over 97% sensitivity, an d 100% specificity as compared to anti-VCA reactivity in an immunofluo rescence assay. In addition, no evidence for cross-reactivity was seen in reactions with members of a panel of human serum specimens of know n reactivity with each of the other known human herpesviruses. Thus, b aculovirus-expressed gp125 should prove a stable platform on which new Epstein-Barr virus-specific serodiagnostic tests can be built.