DEVELOPMENT OF A QUANTAL ASSAY IN PRIMARY SHRIMP CELL-CULTURE FOR YELLOW HEAD BACULOVIRUS (YBV) OF PENAEID SHRIMP

Authors
LU Y TAPAY LM LOH PC BROCK JA GOSE R
Citation
Y. Lu et al., DEVELOPMENT OF A QUANTAL ASSAY IN PRIMARY SHRIMP CELL-CULTURE FOR YELLOW HEAD BACULOVIRUS (YBV) OF PENAEID SHRIMP, Journal of virological methods, 52(1-2), 1995, pp. 231-236
Citations number
6
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
52
Issue
1-2
Year of publication
1995
Pages
231 - 236
Database
ISI
SICI code
0166-0934(1995)52:1-2<231:DOAQAI>2.0.ZU;2-V
Abstract
A 50% tissue culture infectious dose assay (TCID50) using primary cult ure of shrimp lymphoid organ (Oka) cells was developed for the quantit ative titration of yellow-head baculovirus (YBV), a newly isolated vir us of penaeid shrimp. The assay protocol includes the use of Primaria- grade 96-well tissue culture plates to grow the primary lymphoid organ cells of penaeid shrimp. A 15% gill suspension from YBV-infected shri mp was determined to have an infectious virus titer of 5 . 10(5.75) TC ID50/ml. This report represents the first convenient assay protocol us ing cell culture derived from penaeid shrimp to titer a shrimp virus.