Cj. Link et al., GENE-SPECIFIC DNA-REPAIR OF DAMAGE-INDUCED IN FAMILIAL ALZHEIMER-DISEASE CELLS BY ULTRAVIOLET-IRRADIATION OR BY NITROGEN-MUSTARD, Mutation research. DNA repair, 336(2), 1995, pp. 115-121
We have measured gene specific DNA repair in a normal human fibroblast
cell line, and in fibroblast lines from two patients with familial Al
zheimer disease (AD). Cells were treated with either ultraviolet radia
tion (UV) or the chemotherapeutic alkylating agent, nitrogen mustard (
HN2). DNA damage formation and repair were studied in the active dihyd
rofolate reductase (DHFR) gene for the main lesions introduced by each
of these two types of DNA damaging agents. The gene specific repair o
f UV induced cyclobutane pyrimidine dimers in the human DHFR gene was
86% complete in the AD cells after 24 h of repair incubation. This rep
air efficiency was similar to what we and others have found in normal
human fibroblasts. After treatment of the AD cells with HN2, we found
the frequency of HN2 induced lesions in the DHFR gene to be similar to
the frequency in the transcriptionally inactive delta-globin gene. Th
e gene specific repair of HN2 induced lesions in the DHFR gene was com
pleted within 8-24 h in the normal fibroblast line and in the familial
AD line, and the repair kinetics were similar for both cell lines. Th
ese results indicate that familial AD fibroblasts have normal gene spe
cific repair of both UV induced and HN2 induced DNA damage in active g
enes.