The aim of the present study was to specify the experimental condition
s for the use of the polymerase chain reaction (PCR) in the detection
of the pseudorabies virus in boar semen. Boar semen was artificially c
ontaminated by Aujeszky's disease virus and submitted to the PCR. The
seminal plasma fraction of the semen was found inhibitory for PCR. An
incubation of the samples with proteinase K followed by an extraction
of the viral DNA on a glass matrix was shown to be able to remove the
inhibition. The method was simple, rapid and allowed the detection of
around 370 viral DNA sequences per microliter of sample. The detection
of the amplified DNA by a specific enzymatically labelled probe could
improve the sensitivity of the method.