FLOW-CYTOMETRIC QUANTIFICATION OF CD34-PO SITIVE CELLS

In the present study three clinical applications of flow-cytometric qu antification of CD34-positive cells in the field of blood and marrow t ransplantation are evaluated. By daily determination of CD34-positive cells in the peripheral blood after mobilization of stem cells with ch emotherapy or growth-factors it is possible to set the optimal time fo r collection of the transplant material. Thereafter the yield of CD34- positive cells within the harvested material is determined. Finally, t he efficacy of positive CD34 selection using a biotin-avidin column is evaluated. Initial experience is presented here.