EFFECT OF THEOPHYLLINE ADMINISTERED INTRATRACHEALLY AS A DRY POWDER FORMULATION ON BRONCHOSPASM AND AIRWAY MICROVASCULAR LEAKAGE IN THE ANESTHETIZED GUINEA-PIG
D. Raeburn et Vr. Woodman, EFFECT OF THEOPHYLLINE ADMINISTERED INTRATRACHEALLY AS A DRY POWDER FORMULATION ON BRONCHOSPASM AND AIRWAY MICROVASCULAR LEAKAGE IN THE ANESTHETIZED GUINEA-PIG, Pulmonary pharmacology, 7(4), 1994, pp. 243-249
The effect of theophylline (a non-selective phosphodiesterase (PDE) in
hibitor), dosed intratracheally (it) as a dry powder, on histamine- an
d platelet activating factor (Paf)-induced bronchospasm and antigen (o
valbumin, OA)-, histamine- and Paf-induced microvascular leakage (MVL)
in the airways, was studied in the anaesthetized guinea-pig. Bronchos
pasm was measured as the increase in pulmonary inflation pressure (PIP
). MVL was assessed by fluorometric assay of fluorescein isothiocyanat
e dextran (FITC-dextran) content in airway tissues and tracheobronchia
l lavage fluid. OA (200 mu g), histamine (60 nmol) and Paf (4 nmol), a
ll given it, significantly increased MVL by up to 350% over levels in
undosed unchallenged animals. Theophylline (50-500 mu g it, n=5-6) inh
ibited histamine-induced bronchospasm (30% inhibitory dose, ID30: 258/-30 mu g) and Paf-induced bronchospasm (ID30: 190+/-80 mu g). An inhi
bition of 40-50% of maximal bronchospasm was the largest attained. The
ophylline, at approximately the bronchospasm ID30 dose (200 mu g it, n
=4-8), inhibited MVL induced by all agents by 30-80% in airway tissues
and in lavage fluid samples. Theophylline (50-500 mu g it, n=3) produ
ced plasma drug levels of 0.13+/-0.07 to 0.83+/-0.39 mu g/ml 10 min af
ter dosing. Plasma levels were the same 60 min after dosing, suggestin
g retention of theophylline in the airways. The local concentration of
theophylline retained in the airways should be sufficient to inhibit
PDE activity. Direct application of theophylline (arguably by inhibiti
on of the PDE isoforms PDE III, PDE IV and PDE V) thus has significant
antiinflammatory and some bronchodilator effects at very low doses wh
ich should have no systemic toxicity. Theophylline applied as a dry po
wder locally in the airways may thus improve its documented usefulness
in the treatment of asthma.