We performed a prospective study to investigate whether the sonographi
c appearance of the testicular parenchyma changes during maturation of
the testis. One hundred forty-three boys ranging in age from 6 months
to 16 years, and 11 volunteers aged about 30 years, were included in
the study. The echogenicity of each of the 308 normal testes was deter
mined by measuring the average brightness of a histogram over a define
d testicular area. To avoid divergent results due to different setting
s of the device in individual cases, the average brightness of a histo
gram of a standard ultrasound phantom was measured immediately after t
he examination of each testicle. From these 2 measurements, testicular
echogenicity could be calculated independently of individual equipmen
t settings. All testes had a homogeneous echotexture. The echogenicity
of the prepubertal testis was markedly reduced compared to the medium
-level echogenicity of the mature testis (p < 0.001). Testicular echog
enicity increases during puberty, We conclude that testicular echogeni
city increases with maturation. Knowledge of this fact may improve the
diagnostic performance of scrotal ultrasound especially in children.