COMPLETE SEQUENCE OF THE BINARY VECTOR BIN-19

Despite the widespread use of Bin 19 as a vector for plant transformat ion, detailed sequence information on its T-DNA region has only recent ly become available. We now show that the non-T-DNA region, like the T -DNA region, contains several superfluous insertions and find that som e functional elements may not contain optimal sequences. Knowledge of the complete 11777 bp sequence will aid in the construction of excepti onally efficient derivative vectors of approximately half this size. P recise knowledge of restriction sites and removal of unnecessary seque nces will facilitate plasmid manipulations and plant transformation.