STUDIES ON DRUG-METABOLISM USING LC ATMOS PHERIC-PRESSURE CHEMICAL IONIZATION-MS

In this study a column switching system was introduced between the LC/ atmospheric pressure chemical ionization (APCI)-MS interface and HPLC. This method introduced only the objective components to MS without th e need for troublesome pretreatment to remove contaminants from biolog ical samples, effectively cleaning the interface and modifying the ana lytical conditions. After addition to rat plasma. drugs and their pola r metabolites were analyzed simultaneously and identified. The metabol ites were detected after administration of the drugs to rats, and thei r structures were elucidated. Further, we have clarified some characte ristic aspects of the LC/APCI-MS interface.