REAL-TIME DETECTION OF MITOCHONDRIAL INHIBITION AT FROG MOTOR-NERVE TERMINALS USING INCREASES IN THE SPATIAL VARIANCE IN PROBABILITY OF TRANSMITTER RELEASE

Citation
Sd. Provan et Md. Miyamoto, REAL-TIME DETECTION OF MITOCHONDRIAL INHIBITION AT FROG MOTOR-NERVE TERMINALS USING INCREASES IN THE SPATIAL VARIANCE IN PROBABILITY OF TRANSMITTER RELEASE, Neuroscience letters, 185(3), 1995, pp. 187-190
Citations number
24
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
03043940
Volume
185
Issue
3
Year of publication
1995
Pages
187 - 190
Database
ISI
SICI code
0304-3940(1995)185:3<187:RDOMIA>2.0.ZU;2-9
Abstract
The effects of Hg2+, methyl mercury, and flufenamic acid, all of which inhibit mitochondria, were examined at frog motor nerve terminals. Un biased estimates of m (no, of transmitter quanta released), n (no, of functional release sites), p (probability of release), and var, p (spa tial variance in p) were obtained using K+-induced asynchronous neuros ecretion (m, n and p not having the same definitions as with nerve-evo ked release). Transient but significant increases in m, n, p and var, p were found with all three agents. These findings indicate that mitoc hondrial inhibition and release of sequestered Ca2+ can be detected as a real-time increase in var, p. The results also suggest that changes in var, p might be used to differentiate between cellular (membrane) and subcellular (organellar) actions of drugs at the nerve terminal.