A wet condensation heater has been modified to disinfect drain water i
n greenhouses in addition to providing heating and CO2. Various kinds
of microorganisms (bacteria and fungi) and one virus (tobacco mosaic v
irus [TMV]) originating from either pure cultures or substrate extract
s were introduced into the disinfection circuit above the heater. Wate
r was checked downstream for the number of microorganisms surviving th
e heat treatment. Fungi were destroyed after 30 s at 58.9 degrees C, a
nd most of the bacteria except some spore-forming bacteria such as Bac
illus polymyxa were destroyed after 45 s at 65.2 degrees C. Complete e
limination of TMV particles was more difficult to achieve but their nu
mber was greatly reduced, limiting the probability of plant infection.
The microbial density was significantly decreased at relatively low t
emperatures (less than 75 degrees C for 2 min) and so the efficacy of
the heater was attributed to a combined effect of both temperature and
UV irradiation from the flame. Addition of a nonpathogenic microorgan
ism to the substratum is proposed to compensate for the lack of comple
te efficiency observed with high flow rates of drain water. This intro
duced microorganism could occupy the free space and limit an invasion
by a pathogen surviving the treatment.