APPLICATION OF A POLYMORPHIC Y-MICROSATELLITE TO THE DETECTION OF POST BONE-MARROW TRANSPLANTATION CHIMERISM

Authors
LO YMD NOAKES L ROUX E JEANNET M CHAPUIS B FLEMING KA WAINSCOAT JS
Citation
Ymd. Lo et al., APPLICATION OF A POLYMORPHIC Y-MICROSATELLITE TO THE DETECTION OF POST BONE-MARROW TRANSPLANTATION CHIMERISM, British Journal of Haematology, 89(3), 1995, pp. 645-649
Citations number
9
Categorie Soggetti
Hematology
Journal title
British Journal of Haematology → ACNP
ISSN journal
00071048
Volume
89
Issue
3
Year of publication
1995
Pages
645 - 649
Database
ISI
SICI code
0007-1048(1995)89:3<645:AOAPYT>2.0.ZU;2-T
Abstract
A highly sensitive Y-microsatellite amplification system was developed for the detection of post bone marrow transplantation (BMT) chimaeris m. The system is able to detect the DNA equivalent of a single male ce ll in a background of 10(5) female cells. For clinical applications it has the distinct advantage that the polymorphic nature of the PCR pro duct allows the checking of the genuineness of a positive PCR signal r eference to ts allelic type. This extra degree of precision enhances t he accuracy of Y-PCR for clinical diagnosis.