GENOTYPE DISTRIBUTION IN NAGOYA AND NEW GENOTYPE (GENOTYPE 3A) IN JAPANESE PATIENTS WITH HEPATITIS-C VIRUS

We evaluated hepatitis C virus (HCV) genotype distribution among Japan ese patients in the city of Nagoya and the possible existence of any o ther genotype not determined by Okamoto's method. Eighty-five of 93 (9 1.4%) anti-HCV-positive patients had detectable HCV RNA. The genotype of the HCV isolate was determined in 84 of 85 (98.8%) of these HCV RNA -positive patients by Okamoto's method but determination was not possi ble in one (1.2%). Genotype Ib was detected in 58 of the 85 patients ( 68.2%), genotype 2a in 20 (23.5%), genotype 2b in 3 (3.5%), and genoty pe 1b+2a in 3 (3.5%). In the remaining 1 patient in whom the genotype could not be determined, we determined the nucleotide sequence of the core region in HCV RNA extracted from this patient and evaluated it by molecular evolutionary analysis. This HCV isolate was then classified as genotype 3a. These results suggest that genotype 3a is rare among Japanese patients with HCV; thus, when classifying Japaneses isolates, we should take more care because genotype 3a is not determined by cur rent typing systems.