ENOLASE AND THE ARSONOMETHYL ANALOG OF 2-PHOSPHOGLYCERATE

Authors
CHAWLA S DIXON HBF
Citation
S. Chawla et Hbf. Dixon, ENOLASE AND THE ARSONOMETHYL ANALOG OF 2-PHOSPHOGLYCERATE, Journal of enzyme inhibition, 8(4), 1995, pp. 255-259
Citations number
15
Categorie Soggetti
Biology
Journal title
Journal of enzyme inhibition → ACNP
ISSN journal
87555093
Volume
8
Issue
4
Year of publication
1995
Pages
255 - 259
Database
ISI
SICI code
8755-5093(1995)8:4<255:EATAAO>2.0.ZU;2-Q
Abstract
(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the a ction of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. I t is a substrate for yeast enolase (EC 4.2.1.11) with a K-m of 6.5 mM (for 2-phospho-D-glycerate K-m = 0.08 mM). The catalytic constant of t he enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.