DIFFERENTIAL-DIAGNOSIS OF LUNG NEMATODE PARASITES FROM LIVESTOCK BY ELECTROPHORETIC TECHNIQUES

Cutillas C., Espina C., Spakulova M. and Arias, P. 1995. Differential diagnosis of lung nematode parasites from livestock by electrophoretic techniques. International Journal for Parasitology 25: 215-220. The p rotein profile, determined by SDS-PAGE, from different geographical st rains (Slovakia and Spain) of Dictyocaulus filaria and Prorostrongylus rufescens parasitizing Capra hircus and Ovis aries has been assayed. This protein profile has also been comparatively determined in D. vivi parus isolated from lungs of Bos taurus killed in Slovakia, by SDS-PAG E. Protein profiles of D. viviparus and both strains of D. filaria wer e very similar while in P. rufescens a quite different protein profile was found. Furthermore, the isoenzymatic pattern of Lactate Dehydroge nase (LDH) has been studied in two different geographical strains of D . filaria and P. rufescens and in D. viviparus by starch gel electroph oresis. From both strains of D. filaria and from both sexes, the isoen zymatic pattern of LDH was characterized by the presence of five isoen zymes, four anodical and one cathodical. In contrast, males and female s of D. viviparus, showed a different LDH isoenzymatic pattern; males presenting two isoenzymes with anodical and cathodical migration, resp ectively, and females showed only a single isoenzyme with anodical mig ration. Moreover, the electrophoretic mobility of the D. viviparus iso enzymes was different to that of D. filavia. Therefore, LDH has been d esignated as an important diagnostic tool to differentiate between spe cies of genus Dictyocaulus. Finally, the LDH isoenzymatic pattern in P . rufescens (Slovakian and Spanish strain) was identical in both sexes appearing as a single band with cathodical migration.