EFFECT OF CERTAIN CRYOPROTECTANTS ON FREEZING OF BUFFALO SEMEN IN MILK DILUTORS

Post-freezing per cent motile and per cent live sperm varied significa ntly in the presence of different sugars in a milk diluent containing different levels of yolk and glycerol. Optimum concentration of yolk w as 5% and of glycerol 6%. Addition of many sugars, except fructose wit h different combinations of yolk and glycerol did not improve post-tha w motility (PTM) and live sperm. PTM was highest in milk containing 5% yolk +6% glycerol +1.0% fructose. PTM decreased significantly after a ddition of glucose, raffinose, common table sugar and xylose. Combinat ion of higher yolk and glycerol depressed PTM. Per cent live sperm aft er freezing increased slightly after addition of fructose, whereas add ition of other sugars decreased per cent live sperm.