R. Konieczny, PLANT-REGENERATION FROM IMMATURE EMBRYO CULTURE OF TRIFOLIUM-MICHELIANUM SAVI - HISTOLOGICAL OBSERVATIONS ON ADVENTITIOUS SHOOT INDUCTION, Acta Societatis Botanicorum Poloniae, 65(3-4), 1996, pp. 261-266
Whole plant regeneration via organogenesis in immature embryo culture
of Trifolium michelianium Savi. was obtained. The shoots were induced
directly from the hypocotyl or indirectly through callus on EC6 medium
supplemented with 0.5 and 5 mg/l BAP, respectively. Sporadically the
callus formation was preceded by shoot regeneration. Both shoots obtai
ned directly and via callus regenerated into plants when subcultured o
n half-strength Murashige and Skoog (MS) medium, free of growth regula
tors. Histological observations revealed that the shoots were induced
directly from the ring of meristematic tissue which was formed at the
periphery of the hypocotyl or indirectly from the superficial cells of
the callus. Irrespective of the mode of regeneration the shoots were
of multicellular origin.