HETERONUCLEAR 2-DIMENSIONAL AND 3-DIMENSIONAL NMR-STUDIES ON THE R1-R2-R3 DOMAIN OF DROSOPHILA-MELANOGASTER C-MYB PROTEIN - SPIN SYSTEM IDENTIFICATIONS

Advantages of heteronuclear two- and three-dimensional NMR experiments in obtaining better dispersion of peaks in spectra of large protein m olecules have been described. The basic experimental techniques have b een qualitatively presented and their application to a protein of 160 amino acid residues has been described. Several residue-type specific signals have been identified. The analysis of three-dimensional C-13 r esolved H-1-H-1 TOCSY spectra for spin system identifications has been described in some detail.