NUCLEOTIDE-SEQUENCE OF THE REGION BETWEEN CRR AND CYSM IN SALMONELLA-TYPHIMURIUM - 5 NOVEL ORFS INCLUDING ONE ENCODING A PUTATIVE TRANSCRIPTIONAL REGULATOR OF THE PHOSPHOTRANSFERASE SYSTEM

A 4471 bp region between err and cysM on the Salmonella typhimurium ch romosome (49.5 min) has been sequenced. Five ORFs were found within th is region, one of which is likely to be the putative regulatory gene, ptsJ, that corresponds in map position to a gene which when mutated al lows expression of a cryptic Enzyme I of the phosphotransferase system . The deduced amino acid sequence of the encoded protein is similar to those of several open reading frames (ORFs) including ORFT2 of Rhodob acter spheroides with which it is 28% identical throughout most of its length (comparison score of 21 S.D.). PtsJ exhibits a putative, N-ter minal, helix-turn-helix, DNA binding domain that is similar in sequenc e to those in members of the GntR family of transcriptional regulators . Analyses of the sequences of the ORFs encoded within this region are presented.