INTERACTION OF JASMONIC AND ABSCISIC-ACID IN THE CONTROL OF LIPASES AND PROTEASES IN GERMINATING APPLE EMBRYOS

Effects of jasmonic (JA) and abscisic (ABA) acid on the activities of lipases and proteases were studied in cultured embryos excised from ap ple seeds. JA stimulated alkaline (AlkL) and acid (Act) lipases as wel l as proteases hydrolyzing casein, L-cystine-di-beta-naphthylamide (Cy s beta Na), glycyl-glycyl-glycine (GlyGlyGly) and native apple seed pr oteins. ABA inhibited AlkL and protease activities, with the exception of the hydrolysis of native proteins which was stimulated. The activi ty of Act was not affected by ABA. AlkL; was affected by JA and ABA al so in vitro. An antaogonistic interaction between JA and ABA on all st udied enzyme activities, except Cys beta Na hydrolyzing protease, was observed. The positions of JA- and ABA-controlled sites in the metabol ic regulation of the removal of embryonal dormancy in apple seeds are discussed.