HUMAN CALGIZZARIN - ONE COLORECTAL CANCER-RELATED GENE SELECTED BY A LARGE-SCALE RANDOM CDNA SEQUENCING AND NORTHERN BLOT ANALYSIS

A cDNA library was constructed from COLO 205 and 1056 clones randomly selected from this library were partially sequenced. Two hundred and t wo (38.4%) out of 526 independent genes had more than 80% similarity t o the genes reported in GenBank. In Northern blot analysis, 96 out of 98 genes were shown to be expressed at the same level in colon and lun g carcinoma cell lines and control fibroblasts. Only two clones, inclu ding human synovial phospholipase A-2 and a homologue to rabbit calgiz zarin, were expressed at different levels among these cell lines. The full sequence of human calgizzarin was determined and its expression w as remarkably elevated in colorectal cancers compared with that in nor mal colorectal mucosa.