CARBOXYFLUORESCEIN AND BIOTIN NEUROMEDIN-C ANALOGS - SYNTHESIS AND APPLICATIONS

Two neuromedin C (NC) analogues were constructed by Fmoc synthesis and in situ coupling of 4(5)-carboxyfluorescein or biotin to the N-termin us. Both displayed full agonism in an amylase release assay and cross- reacted fully with a NC-specific antiserum. Biotin NC functioned in a strepavidin-capture ELISA. Carboxyfluorescein NC was used to probe rec eptor localization in rat stomach. Specific NC binding sites, which di d not interact with substance P, angiotensin I, or neurokinin A, were labeled in the antrum. Identity of NC binding sites was confirmed by m icroautoradiography. The specifically labeled cells were all found in the lamina propria and at Least some of cells were identified as eosin ophils.