T. Josephhorne et al., DEFECTIVE STEROL DELTA(5(6))DESATURASE AS A CAUSE OF AZOLE RESISTANCEIN USTILAGO-MAYDIS, FEMS microbiology letters, 127(1-2), 1995, pp. 29-34
Resistance to azole antifungals in Ustilago maydis was associated with
a leaky defect in sterol Delta(5(6))desaturase. This defect resulted
in reduced accumulation of 14 alpha-methylergosta-24(28)-diene-3 beta,
6 alpha-diol and an increase in the proportion of 14 alpha-methylfecos
terol in treated cells when compared to the parent strain. The results
demonstrate the importance of this mechanism in pathogenic fungi.